Sodium Salt

1. Heparin was originally extracted from the liver, and is now mostly extracted from the intestinal mucosa of pigs, sheep, cattle and other animals, and can be extracted from the lungs and liver. Put the fresh intestinal mucosa into the reaction pot, add sodium chloride at 3%, and adjust the pH to 9.5 with 40% sodium hydroxide. Gradually heat up to 60-65 ° C within half an hour, continue to hold heat and stir for 2h, then heat up to 95 ° C, maintain for 10min, cool to below 50 ° C and filter. The strong basic quaternary ammonium salt anionic resin was added into the filtrate, stirred and adsorbed for 8 hours, and the liquid was left to stand. The next day, the upper liquid was siphoned off, the resin was rinsed with water to clarify the top liquid, drained, stirred with twice the amount of 1.4M sodium chloride for 2 hours, drained, and the resin was washed once again without twice the amount of 1.4M sodium chloride. Then, the resin was eluted twice with 3M sodium chloride, drained, combined the filtrate, added an equal amount of 95% ethanol precipitation solution, collected precipitation, dehydration and drying with acetone to obtain coarse products. Coarse products are dissolved in 1% sodium chloride, pHGCF 1.7-1.8 adjusted with hydrochloric acid, and filtered. Adjust pH to 11 with 5N sodium hydroxide, add 30% concentration hydrogen peroxide for decolorization, and filter. The filtrate was adjusted to pH 6 with hydrochloric acid and precipitated with an equal amount of 95% ethanol. The precipitated substance is washed with acetone and dried, which is heparin.

2. Enzymolysis - resin method

3. Salt-resin method