Glycine
Glycine is widely used as a buffer for a variety of immunological applications. It is frequently used in the preparation of TG Buffers (Tris-Glycine) where the buffer is used as a running and/or transfer buffer for polyacrylamide gel electrophoresis and western blotting. Glycine (100 mM, pH 2.0-2.5) is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase.
Glycine is a component of Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.
Glycine (Gly), also known as glycine, is a non-essential amino acid with the formula C2H5NO2. Glycine is a constituent amino acid of the endogenous antioxidant reduced glutathione, which is often supplemented exogenously during severe stress in the body, and is sometimes called a semi-essential amino acid. [1] Glycine is one of the simplest amino acids. [4]
Solid glycine is a white to off-white crystalline powder, odorless, non-toxic [2]. Soluble in water and almost insoluble in ethanol or ether. Used in the pharmaceutical industry, biochemical testing and organic synthesis, is the simplest structure in the amino acid series, non-essential amino acids in the human body, in the molecule has both acidic and basic functional groups, ionization in water, with strong hydrophilicity, but belongs to non-polar amino acids, soluble in polar solvents, and difficult to dissolve in non-polar solvents [3], and has a high boiling point and melting point, through the adjustment of aqueous solution acidity and alkalinity can make glycine present different molecular forms.
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